The bioburden membrane filtration method is a microbiology test. the packaging material is a Laminate tube and aluminum tube. it is suitable for finishing products, bacterial growth, or fungal growth.
Select twenty samples at random from the sample lot
Accessories required for testing
- 20 sample of tubes
- 1% sterile peptone
- Sterile soybean casein digest agar plate
- Sterile membrane filter
- Sabourauds chloramphenicol agar
Procedure
- Aseptically place 4 ml of sterile 0.1% peptone in each of the 10 samples of the aluminium tube from the open end, fold the end, and shake the tube thoroughly.
- Collect the rinsing solution from each tube and filter the pooled solution through a sterile membrane filter.
- Place the membrane filter aseptically on the surface of a sterile pre-incubated casein digest agar plate
- Incubated the above plate at 300c – 350c for day in inverted position.
- Count the colonies observed on the membrane filter and report the total bacterial count as ‘X ‘ cfu/ 10 units.
- Repeat the above steps for the fungal count by using Sabourauds chloramphenicol agar and incubate the plate 200c – 250c for 5 days
- Count the colonies observed on the membrane filter and report the total fungal counts as ‘y’ cfu/10 units
I am Maneesh Maurya a professional pharmaceutical blogger from India having rich experience in the pharmaceutical Quality control field.
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